Protein fishing from single live cells.
J Nanobiotechnology. 2018 Sep 11;16(1):67
Authors: Shekaramiz E, Doshi R, Wickramasinghe HK
Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics.
PMID: 30205820 [PubMed – in process]